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T. Wilkinson ; A. Douglas | 1995Pea aphids (Acyrthosiphon pisum) whose symbiotic bacteria were disrupted with the antibiotic chlortetracycline over the first 5 days after birth (i.e, aposymbiotic aphids), were able to feed from the host plant Vicia faba, as indicated by the re[...]
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T. Wilkinson ; D. Ashford ; J. Pritchard ; A. Douglas | 1997Pea aphids, Acyrthosiphon pisum, containing their symbiotic bacteria (untreated aphids) and experimentally deprived of their bacteria by treatment with the antibiotic rifampicin (antibiotic-treated aphids) were reared on the plant Vicia faba, Th[...]
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T. Wilkinson ; A. Douglas | 1998The symbiotic bacteria Buchnera in aphids are borne in cells, called bacteriocytes, in the insect haemocoel. The number and median volume of bacteriocytes in pre-reproductive adult insects varied significantly among 14 parthenogenetic clones of [...]
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D. Adams ; A. Douglas | 1997To explore the effect of rearing-plant species on the contribution of the symbiotic bacterium, Buchnera, to aphid performance. larvae of Aphis fabae that contained the bacteria (symbiotic aphids) and larvae experimentally deprived of the bacteri[...]
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D. Ashford ; W. Smith ; A. Douglas | 2000The natural diet of aphids, plant phloem sap, generally contains high concentrations of sucrose. When pea aphids (Acyrthosiphon pisum) were fed on chemically defined diets containing sucrose radiolabelled in the glucose or fructose moiety, 2 to [...]
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L. Birkle ; A. Douglas | 1999Genetic diversity in the pea aphid Acyrthosiphon pisum was investigated by a restriction fragment length polymorphism (RFLP) analysis of three maternally inherited genomes (mitochondrial DNA and plasmids of the symbiotic bacteria Buchnera). Twen[...]
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J. Abisgold ; S. Simpson ; A. Douglas | 1994A recently developed framework was applied to investigate the responses of newborn pea aphids, Acyrthosiphon pisum (Harris) (Homoptera: Aphididae), to simultaneous variations ip dietary sucrose and amino acid levels. The location of functional '[...]
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A. Douglas ; A. Douglas, Editeur scientifique | 1990
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C. Tosh ; K. Walters ; A. Douglas | 2001
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B. Raymond ; J. Searle ; A. Douglas | 2001Models of sympatric speciation for phytophagous insects are based on the premise that ecological specialization on different host plants can indirectly result in significant reproductive isolation. A candidate example of host plant shift is prov[...]
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N. Humphreys ; A. Douglas | 1997The population of symbiotic Buchnera bacteria in parthenogenetic females of the yea aphid Acyrthosiphon pisum mas determined by quantitative hybridization of a DNA probe (gro ESL) to aphid homogenates. The aphids bore 1 x 10(7) to 2 x 10(7) bact[...]
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T. Wilkinson ; A. Douglas | 1998Plant penetration by the stylets of six clones of the pea aphid, Acyrthosiphon pisum, on Vicia faba (acceptable to all clones) and Pisum sativum (acceptable to 3/6 clones) was investigated by the DC electrical penetration graph technique. In a 1[...]
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A. Douglas | 1997Twenty-nine clones of Aphis fabae Scopoli (Aphididae: Homoptera) were isolated into laboratory culture from the primary host plants Euonymus europaeus and Viburnum opulus and the secondary host plants Arctium lappus, Impatiens glandulifera, Trop[...]
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A. Douglas | 1996Pea aphids (Acyrthosiphon pisum) treated at birth with the antibiotic, rifampicin, to disrupt the symbiotic bacteria (i.e, aposymbiotic aphids) grow very slowly, The growth of embryos in the aposymbiotic aphids was particularly depressed, such t[...]
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